Evaluation of the subsets of lymphocytes and their activated status in patients with myelodysplastic syndrome.
- Author:
Jun YANG
1
;
Chun WANG
;
Kuang-Cheng XIE
;
Shi-Ke YAN
;
Yan-Rong GAO
;
Qi CAI
;
You-Wen QIN
;
Li-Ping WAN
;
Yu CAI
Author Information
1. Department of Hematology, The Shanghai First People Hospital, Shanghai 200080, China. yangjuan74@sina.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Antigens, CD;
immunology;
Antigens, Differentiation, T-Lymphocyte;
immunology;
B-Lymphocytes;
immunology;
CD3 Complex;
immunology;
Female;
HLA-DR Antigens;
immunology;
Humans;
Killer Cells, Natural;
immunology;
Lectins, C-Type;
Lymphocyte Activation;
immunology;
Male;
Middle Aged;
Myelodysplastic Syndromes;
immunology;
T-Lymphocyte Subsets;
immunology
- From:
Journal of Experimental Hematology
2006;14(4):708-713
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the clinical significance of the amount and activated status of T cell subsets, B cells, NK cells in peripheral blood from patients with myelodysplastic syndrome (MDS). The proportion of T cells, B cells, NK cells in peripheral blood from 30 patients with MDS and their surface activation markers of CD28, CD45RA, CD45RO, CD69, HLA-DR were analyzed by flow cytometry. Twenty-two patients were in the low risk group (RA + RAS) while eight patients were in the high risk group (RAEB + RAEBT). The result showed that the amounts of T cells (CD3+ cells) in peripheral blood from patients with MDS were lower than those in control group. The amounts of naive CD4+ cells (CD4+ CD45RA+ cells) in MDS patients were lower than those in control. The expression rates of early activation marker (CD69) and late activation marker (HLA-DR) on CD3+ cells in MDS patients were significantly higher than those in control. The abnormalities of the immunologically competent cells were mainly observed in the low risk group (RA + RAS), and were characterized by the high expression rates of CD69+ and HLA-DR+ on CD3+ cells, the decrease of B cell amounts. The amount abnormalities of T cell subsets were mainly observed in high risk group (RAEB + RAEBT), and were characterized by the decrease of CD3+ cells and CD3+ CD4+ CD8- cells (Th cells) amounts without high expression of the CD69 and HLA-DR, the decrease of NK cells amounts. It is concluded that there are the abnormalities of T cell subsets and function in the patients with MDS and may change with disease progression, so the measurement of amount and activated status of T cell subsets in peripheral blood from MDS patients can have predictive role for diagnosis of disease progression and guide of therapy.
