Immunophenotypic characteristics of children with acute lymphoblastic leukemia carrying TEL-AML1 fusion gene.
- Author:
Yi LIU
1
;
Zhi-Gang LI
;
Wei ZHAO
;
Bei LI
;
Wen-Yu GONG
;
Min-Yuan WU
Author Information
1. Hematological Center, Beijing Children Hospital, Capital Medical University, Beijing 100045, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD20;
analysis;
Burkitt Lymphoma;
genetics;
immunology;
CD13 Antigens;
analysis;
Child;
Child, Preschool;
Core Binding Factor Alpha 2 Subunit;
genetics;
Female;
HLA-DR Antigens;
analysis;
Humans;
Immunophenotyping;
Infant;
Male;
Oncogene Proteins, Fusion;
genetics
- From:
Journal of Experimental Hematology
2006;14(4):714-716
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the immunological and other clinical characteristics in TEL/AML1+ childhood B-acute lymphoblastic leukemia (B-ALL), immunophenotyping was performed with three-color flow cytometry, and the expression of TEL-AML1 fusion gene was detected with nested RT-PCR. Diagnosis was made according to FAB and MIC criteria. The results showed that (1) among 119 children with B-ALL, 22 (18.5%) were TEL-AML1 positive and classified as L2 morphological subtype. In TEL-AML1+ group, positive rate and score of PAS, which were 65% and 121 respectively, were all higher than that of TEL-AML1- group (P < 0.05); (2) compared with TEL-AML1- group, no significant difference was found in age, gender, white cell count and blasts count in peripheral blood of TEL-AML1+; (3) in TEL-AML1+ group, 21 out of 22 (95.5%) were common ALL, as compared with TEL-AML1- group, the positive rate of CD13 was higher (59.1%, 13/22) and the positive rate of CD20 was lower (22.7%, 5/22) than that in TEL-AML1- group, respectively (P < 0.05), and the mean fluorescence index of CD10 and HLA-DR significantly increased to 92.80 and 53.61, respectively (P < 0.05). It is concluded that TEL-AML1 rearrangement is a frequent molecular abnormality in childhood ALL. Leukemic blasts with this anomaly have special immunophenotypic characteristics. These characteristics may be useful in detection of minimal residual leukemia.
