In vitro effect of all-trans retinoic acid on cell adhesion molecule expression and adhesion capacity of bone marrow stromal cells in patients received peripheral blood stem cell transplantation.
- Author:
Cheng CHANG
1
;
Xing-Hua CHEN
;
Pei-Yan KONG
;
Xian-Gui PENG
;
Dong-Feng ZENG
;
Wen-Bo YANG
;
Xue LIANG
;
Lin LIU
;
Hong LIU
;
Qing-Yu WANG
Author Information
1. Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antigens, CD34;
Antineoplastic Agents;
pharmacology;
Bone Marrow Cells;
metabolism;
pathology;
Cell Adhesion;
drug effects;
Cell Adhesion Molecules;
biosynthesis;
genetics;
Child;
Coculture Techniques;
Hematologic Neoplasms;
metabolism;
pathology;
therapy;
Humans;
Middle Aged;
Peripheral Blood Stem Cell Transplantation;
Stromal Cells;
metabolism;
pathology;
Tretinoin;
pharmacology;
Tumor Cells, Cultured;
Vascular Cell Adhesion Molecule-1;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2006;14(4):768-772
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to evaluate the effect of all-trans retinoic acid (ATRA) on cell adhesion molecule expression and adhesion capacity of bone marrow stromal cells (BMSC) in patients after conditioning treatment for peripheral blood stem cell transplantation (PBSCT). BMSC of 27 patients before and after conditioning treatment for PBSCT were cultured in vitro. After treated with ATRA at 0.01, 0.1, or 1 micromol/L, expression of intercellular adhesion molecule-1 (ICAM-1) protein and vascular adhesion molecule-1 (VCAM-1) protein were detected by flow cytometry, and soluble ICAM-1 (sICAM-1) protein was determined by using radioimmunoassay. Then BMSC was co-cultured with CD34+ cells, and adhesion rate of BMSC to CD34+ cells was measured. The results showed that after pretreatment with conditioning regimen for PBSCT, the expressions of ICAM-1 and VCAM-1 proteins in BMSC and the expression level of sICAM-1 protein in supernatant of BMSC culture were down-regulated, and the adhesion rate of BMSC to CD34+ cells was decreased, after administration of ATRA, the expression of ICAM-1 protein in BMSC, sICAM-1 protein in culture medium and adhesion rate of BMSC to CD34+ cells all increased significantly, but expression of VCAM-1 protein changed no significantly. It is concluded that the ATRA can partly restore adhesion function of BMSC injured by pretreatment for PBSCT and contribute to hematopoietic reconstitution.
