Impact of rhG-CSF on sphingosine 1-phosphate concentration in blood plasma of donors.
- Author:
Wen-Rong HUANG
1
;
Li-Sheng WANG
;
Hai-Feng DUAN
;
Chun-Ji GAO
;
Zhuo-Zhuang LU
;
Hua WANG
;
Wan-Ming DA
Author Information
1. Department of Hematology, General Hospital of Chinese PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Blood Donors;
Granulocyte Colony-Stimulating Factor;
therapeutic use;
Hematopoietic Stem Cell Mobilization;
Humans;
Lysophospholipids;
blood;
RNA, Messenger;
blood;
Recombinant Proteins;
Sphingosine;
analogs & derivatives;
blood
- From:
Journal of Experimental Hematology
2006;14(4):783-786
- CountryChina
- Language:Chinese
-
Abstract:
Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Recombinant human granulocyte-colony-stimulating factor (rhG-CSF) have been used widely in peripheral blood stem/progenitor cell mobilization. This study was aimed to investigate the effects of rhG-CSF on S1P concentration in plasma of donors. The peripheral blood mononuclear cells and blood plasma were collected from 8 peripheral blood progenitor cell donors before mobilization and on the fifth day of mobilization with rhG-CSF. The SphK mRNA expression of blood mononuclear cells were detected by RT-PCR. The changes of S1P concentration were assayed with SphK enzyme catalyzed reaction. The results showed that both kinds blood mononuclear cells collected before and after rhG-CSF mobilization expressed SphK mRNA. The S1P concentration is low in donor's plasma both before and after mobilization with rhG-CSF, and there was no markedly change of S1P concentration in plasma before and after mobilization (P > 0.05). In conclusion, mobilization with rhG-CSF does not impact S1P concentration in donors' plasma.
