Effects of rhG-CSF mobilization on immunological properties of grafts from peripheral blood and bone marrow.
- Author:
Xiang-Yu ZHAO
1
;
Ying-Jun CHANG
;
Xiao-Jun HUANG
Author Information
1. Institute of Hematology and People Hospital, Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Blood Donors;
Bone Marrow Transplantation;
immunology;
Dendritic Cells;
immunology;
Female;
Graft vs Host Disease;
etiology;
Granulocyte Colony-Stimulating Factor;
immunology;
therapeutic use;
Hematopoietic Stem Cell Mobilization;
Hematopoietic Stem Cells;
immunology;
Humans;
Interferon-gamma;
biosynthesis;
Interleukin-4;
biosynthesis;
Male;
Middle Aged;
Peripheral Blood Stem Cell Transplantation;
Recombinant Proteins;
T-Lymphocytes;
immunology
- From:
Journal of Experimental Hematology
2006;14(4):787-790
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the difference of immunological properties between recombination human granulocyte colony-stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB) and rhG-CSF primed bone marrow grafts (G-BM). The lymphocyte proliferation ability and the quantities of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) secreted by T cells were determined by using MTT assays and sandwich ELISA; T cell subgroups, dendritic cells (DC), monocytes and the expression of CD28 costimulatory molecules on T cells were determined by multicolor flow cytometry. The results showed that the absolute numbers of lymphocytes, monocytes, CD3+, CD4+ and CD8+ T cells as well as DC1 and DC2, the ratios of CD4/CD8 in G-PB were significantly higher than those in G-BM, respectively (P < 0.001). T cell proliferation ability was significantly higher in G-PB than that in G-BM (P < 0.05). The quantities of IFN-gamma and IL-4 secreted by T cells per microliter of G-PB was significantly higher than those of G-BM, the ratios of IL-4/IFN-gamma were significantly lower in G-PB than that in G-BM (P < 0.001). As compared with G-BM, the ratio between DC2 and T-lymphocyte was significantly low in G-PB (P < 0.01), whereas the percentage and overall expression of CD28 on CD4+ and CD8+ cells were significantly high in G-PB (P < 0.05). It is concluded that T cell hyporesponsiveness of G-PB and G-BM induced by rhG-CSF in vivo were confirmed to be different, and the difference of immunological properties between G-PB and G-BM may explain the lower incidence of GVHD and lower relapse after G-BM and G-PB transplantation respectively.
