Leukemia-associated immunophenotypes in 415 childhood and adult patients with B lineage acute lymphoblastic leukemia by multiparametric flow cytometry analysis.
- Author:
Yan-Rong LIU
1
;
Shan-Shan CHEN
;
Yan CHANG
;
Jia-Yu FU
;
Le-Ping ZHANG
;
Hui WANG
;
Ling-Di LI
;
Hong-Hu ZHU
;
Gui-Lan LIU
;
Dao-Pei LU
;
Xiao-Jun HUANG
Author Information
1. Institute of Hematology, Peking University, Beijing 100044, China. yanrongliu@yahoo.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Antigens, CD34;
analysis;
B-Lymphocytes;
immunology;
Burkitt Lymphoma;
classification;
immunology;
pathology;
Cell Lineage;
Female;
Flow Cytometry;
methods;
Humans;
Immunophenotyping;
Male;
Middle Aged;
Neoplasm, Residual;
diagnosis;
Neprilysin;
analysis;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
classification;
immunology;
pathology
- From:
Journal of Experimental Hematology
2006;14(5):853-857
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the significance of FCM in minimal residual disease (MRD) detection, the immunophenotyping and leukemia-associated immunophenotypes (LAIP) of leukemia cells from 273 adult and 142 childhood patients with B lineage acute lymphoblastic leukemia (B-ALL) were detected by four to six antibody combinations of 4-color CD45/SSC gating multiparametric flow cytometry (FCM). The results showed that the B-ALL patients could be classified into 4 subtypes based on different expression CD34 and CD10: subtype I (CD34(+)/CD10(-)), subtype II (CD34(+)/CD10(+)), subtype III (CD34(-)/CD10(+)), subtype IV (CD34(-)/CD10(-)). The LAIP was observed in 100% and 92% patients of subtype I and subtype II, respectively, whereas only 79.2% in subtype III. The incidence of LAIP in total B-ALL cases was 90% by using the antibodies detected in this investigation. There was no significantce different for incidence of LAIP between adult and pediatric patients. LAIP was observed in 77.6% of patients by labeling only CD34/CD10/CD19/CD45 4-color antibody combination. It is concluded that in 90% of childhood and adult B-ALL patients LAIP can be found, which suits MRD detection by multiparameter flow cytometry.
