Detection of EML4-ALK fusion gene in non-small cell lung cancer and its clinicopathologic correlation.
- Author:
Shan ZHONG
1
;
Hai-ping ZHANG
;
Jie ZHENG
;
Dong-yu BAI
;
Li FU
;
Pei-qiong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; pathology; surgery; Adult; Aged; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; pathology; surgery; Female; Humans; Lung Neoplasms; genetics; metabolism; pathology; surgery; Male; Middle Aged; Oncogene Proteins, Fusion; metabolism; Sex Factors; Smoking; Young Adult
- From: Chinese Journal of Pathology 2013;42(4):252-256
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the frequency of EML4-ALK fusion gene in non-small-cell lung cancer (NSCLC) patients, and its correlation with clinicopathologic features.
METHODSReal-time PCR was used to detect the presence of EML4-ALK fusion gene in 268 cases of NSCLCs using paraffin-embedded tissue samples(among which 164 samples were re-validated by Sanger sequencing). Related clinicopathological correlation was analyzed.
RESULTSEML4-ALK fusion gene was found in 4.1% (11/268) of the cases. One hundred and sixty four samples were verified by Sanger sequencing, and the overall coincidence of the results of two methods (Sanger sequencing and Real-time PCR) was 100%. Female patients (5.9%, 5/85), ≤ 60 years of age (4.3%, 6/140), non-smokers (6.8%, 8/118) and adenocarcinomas (7.6%, 10/132) had a higher mutation rate than that in male patients (3.3%, 6/183), > 60 years of age (4.0%, 5/124), smokers (1.6%, 2/132) and squamous cell carcinomas (1.3%, 1/79), although no statistical significance in age (P = 0.918), gender (P = 0.503), smoking history (P = 0.092) and histological type (P = 0.094).
CONCLUSIONSChinese NSCLC patients have a 4.1% detection rate of EML4-ALK fusion gene in the tumor tissues. Female, non-smoker and adenocarcinoma histological subtype tend to be associated with a higher rate of EML4-ALK gene fusion.
