Standard protocol of ALK fusion gene assessment by fluorescent in situ hybridization in non-small cell lung cancer.
- Author:
Lei GUO
1
;
Shan ZHENG
;
Yong-qiang XIE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; surgery; Female; Gene Fusion; Humans; In Situ Hybridization, Fluorescence; Lung Neoplasms; genetics; metabolism; surgery; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; metabolism; Paraffin Embedding; Receptor Protein-Tyrosine Kinases; genetics; metabolism; Retrospective Studies; Young Adult
- From: Chinese Journal of Pathology 2013;42(8):530-533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the standard protocol for anaplastic lymphoma kinase (ALK) fusion gene assessment by fluorescent in situ hybridization (FISH) in non-small cell lung cancer (NSCLC).
METHODSTissue specimens of NSCLC cases were retrospectively collected from Jan. 2011 to July 2012. ALK fusion gene was examined by FISH using break-apart ALK gene probes (Vysis company). The identification of ALK fusion gene was determined by fluorescent signals under a fluorescence microscope.
RESULTSOne hundred and forty-six eligible NSCLC tumor specimens were tested for ALK fusion gene by FISH. The specimens included 110 cases (75.4%) of surgically-removed tissues, 11 cases (7.5%) of biopsy, 19 cases (13.0%) of lymph node and 6 cases (4.1%) of other metastatic tissues. The positivity of ALK fusion gene was 8.9% (13/146).
CONCLUSIONSThe assessment of ALK fusing gene by FISH using standard protocol for formalin-fixed, paraffin-embedded (FFPE) tissue is feasible. The protocol can used to test in surgically-removed tissues, biopsies, metastatic lymph nodes and other metastastic specimens.
