Morphology and microRNA expression profiles of drug-resistant cells in hepatocellular carcinoma.
- Author:
Li-juan ZHUO
1
;
Hong CHEN
;
Min-xia WU
;
Mei-qin GAO
;
Shui-ping CHEN
;
Ai-min HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carboplatin; pharmacology; Carcinoma, Hepatocellular; genetics; pathology; ultrastructure; Cell Line, Tumor; Doxorubicin; pharmacology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Gene Expression Profiling; Humans; Liver Neoplasms; genetics; pathology; ultrastructure; MicroRNAs; genetics; metabolism; Mitomycin; pharmacology; Oligonucleotide Array Sequence Analysis
- From: Chinese Journal of Pathology 2013;42(9):604-608
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare morphological differences of three drug-resistant hepatocellular carcinoma (HCC) cell subclones (Huh-7/ADM, Huh-7/CBP, Huh-7/MMC) and their parental Huh-7 cell line, to analyze differential microRNA (miRNA) expression profiles in these cells and, finally to screen for the abnormal expressed miRNAs in drug-resistant HCC cells.
METHODSCellular morphology was observed by histology and transmission electron microscopy. MiRNA microarray was used to analyze the differential miRNA expression profiles in these cells (Huh-7, Huh-7/ADM, Huh-7/CBP, Huh-7/MMC) followed by real time quantitative PCR validation.
RESULTSThe drug-resistant cells had more intracytoplasmic organelles and were larger in size along with increased cytological pleomorphism than the parental Huh-7 cells. Compared with the parental Huh-7 cells, 32 simultaneously up-regulated and 22 down-regulated miRNAs were found in three drug-resistant cells. Up-regulation of miR-15a, miR-16, miR-27b, miR-30b, miR-146a, miR-146b-5p, miR-181a, miR-181d and miR-194 was verified by RT-qPCR.
CONCLUSIONDrug-resistant HCC cells have abnormal expressed miRNAs, which may be explored to further investigate the association of miRNA expressions with multidrugs resistance in HCC.
