Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin.

Rifki Febriansah; Dyaningtyas Dewi Pamungkas Putri; Sarmoko; Nunuk Aries Nurulita; Edy Meiyanto; Agung Endro Nugroho

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Hesperidin as a preventive resistance agent in MCF-7 breast cancer cells line resistance to doxorubicin.

Author: Rifki FEBRIANSAH ^{1
,

2} ; Dyaningtyas Dewi Pamungkas PUTRI ³ ; Sarmoko ⁴ ; Nunuk Aries NURULITA ⁴ ; Edy MEIYANTO ³ ; Agung Endro NUGROHO ³
Author Information

1. Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia
2. Pharmacy study programme, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia.
3. Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia.
4. Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia
Publication Type:Journal Article
Keywords: Apoptosis; Doxorubicin; Hesperidin; MCF-7/Dox cells line; Pgp expression
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(3):228-233
CountryChina
Language:English
Abstract:
OBJECTIVETo evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7/Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin.
METHODSThe cytotoxic properties, 50% inhibition concentration (IC50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7/Dox) cells were determined using MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp.
RESULTSSingle treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC50 value of 11 µmol/L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI>1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expressions level when combined with doxorubicin in low concentration.
CONCLUSIONSHesperidin has cytotoxic effect on MCF-7/Dox cells with IC50 of 11 µmol/L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Co-chemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.