Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels.

Prawez Alam; Aftab Alam; Md Khalid Anwer; Saleh I Alqasoumi

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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels.

Author: Prawez ALAM ¹ ; Aftab ALAM ¹ ; Md Khalid ANWER ² ; Saleh I ALQASOUMI ³
Author Information

1. Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
2. Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
3. Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia.
Publication Type:Journal Article
Keywords: Citrus; HPTLC; Hesperidin
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(4):262-266
CountryChina
Language:English
Abstract:
OBJECTIVETo develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.
METHODSThe method was carried out in aluminum-backed silica gel 60 F254 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.
RESULTSA compact band was obtained for hesperidin at Rf value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.
CONCLUSIONSThe method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.