Anti-HBV effect of nucleotide analogues on mouse model of chronic HBV infection mediated by recombinant adeno-associated virus 8.
- Author:
Guojing WANG
1
;
Gang WANG
;
Xiaoyan DONG
;
Wenhong TIAN
;
Jie YUCHI
;
Guochao WEI
;
Hong MENG
;
Xiaobing WU
Author Information
1. Institute of Basic Medical Science, Shandong Academy of Medical Science, College of Life Science, University of Jinan, Jinan 250000, Shandong, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
pharmacology;
Dependovirus;
genetics;
metabolism;
Disease Models, Animal;
Genetic Vectors;
Genome, Viral;
Guanine;
analogs & derivatives;
pharmacology;
Hepatitis B Antibodies;
blood;
Hepatitis B virus;
genetics;
physiology;
Hepatitis B, Chronic;
virology;
Lamivudine;
pharmacology;
Mice;
Mice, Inbred C57BL;
Nucleotides;
pharmacology;
Transduction, Genetic;
Virus Replication
- From:
Chinese Journal of Biotechnology
2013;29(1):95-106
- CountryChina
- Language:Chinese
-
Abstract:
We evaluated the anti-HBV effects of nucleotide analogues, Entecavir (ETV) and Lamivudine (LAM) targeting mouse model of HBV persistent infection with recombinant adeno-associated virus 8 carrying 1.3 copies of HBV genome (rAAV8-1.3HBV). Ninety percent (27 of 30 mice) of rAAVS-treated mice were chosen as mouse model. Four groups were orally administrated with different doses of ETV (1 mg/(kgd) or 0.1 mg/(kgd)) and LAM (500 mg/(kgd) or 100 mg/(kgd)) once a day for 10 days. The other two groups were set as normal saline treated and untreated control. We detected the levels of HBV DNA, HBeAg and HBsAg in sera at different time. Results indicate that HBV DNA level decreased significantly (P < 0.05) in drug-treated groups compared with normal saline group after drug administration. Fifteen days after the drug withdrawal, HBV DNA level rebounded back obviously (P < 0.05) in groups with low doses of ETV and LAM. However, there was no apparent change of HBeAg and HBsAg in the whole process among all groups. These results showed that our model could reflect the anti-viral effect of nucleotide analogues. This model can be a useful and convenient tool for anti-HBV drug discovery.
