Temperature-switched high-efficiency D-lactate production from glycerol.
- Author:
Kangming TIAN
1
;
Li ZHOU
;
Xianzhong CHEN
;
Wei SHEN
;
Guiyang SHI
;
Suren SINGH
;
Fuping LU
;
Zhengxiang WANG
Author Information
1. School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
- Publication Type:Journal Article
- MeSH:
Aerobiosis;
Anaerobiosis;
Escherichia coli;
genetics;
metabolism;
Fermentation;
Glycerol;
metabolism;
L-Lactate Dehydrogenase;
metabolism;
Lactic Acid;
biosynthesis;
Promoter Regions, Genetic;
genetics;
Temperature
- From:
Chinese Journal of Biotechnology
2013;29(1):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.
