Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis.
- Author:
Zhiyao WANG
1
;
Yubin MA
;
Runzhi MU
;
Changjiang SUN
;
Dongyuan ZHANG
;
Yongfei WANG
Author Information
1. Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.
- Publication Type:Journal Article
- MeSH:
Heavy Ions;
Lipids;
biosynthesis;
Microalgae;
growth & development;
metabolism;
radiation effects;
Mutagenesis;
genetics;
Mutation;
genetics;
Stramenopiles;
genetics;
metabolism;
radiation effects
- From:
Chinese Journal of Biotechnology
2013;29(1):119-122
- CountryChina
- Language:Chinese
-
Abstract:
Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
