Direct secretory expression of active microbial transglutaminase in Pichia pastoris.
- Author:
Pengfei LI
1
;
Hongbing SUN
;
Lijin YOU
;
Fuyu GONG
;
Zao CHEN
;
Ailian ZHANG
;
Taicheng ZHU
Author Information
1. College of Life Science and Agriculture, Hainan University, Haikou 570228, Hainan, China 2 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China.
- Publication Type:Journal Article
- MeSH:
Fermentation;
Genetic Vectors;
genetics;
Pichia;
enzymology;
genetics;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
Streptomyces;
enzymology;
Transglutaminases;
biosynthesis;
genetics
- From:
Chinese Journal of Biotechnology
2013;29(2):180-188
- CountryChina
- Language:Chinese
-
Abstract:
Direct secretory expression of active microbial transglutaminase (MTG) using heterologous hosts is a promising strategy, although its production level still needs to be improved for industrial production. Pichia pastoris is one of the most efficient expression systems developed in recent years. In this study, secretory expression of active MTG was successfully achieved by co-expressing the pro sequence and mature MTG genes in P. pastoris. Furthermore, we optimized the copy number of pro/MTG expression cassettes and the fermentation conditions. MTG production level reached 7.3 U/mL in 1-liter fermentor through high density fermentation, providing the feasiblity for industrial scale preparation of MTG.
