Effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca.
- Author:
Jing WU
1
;
Keke CHENG
;
Wenying LI
;
Jie FENG
;
Jian'an ZHANG
Author Information
1. National and Provincial Joint Key Laborary of Coal Science and Technology of Nurturing Base, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
- Publication Type:Journal Article
- MeSH:
Acetic Acid;
chemistry;
Butylene Glycols;
metabolism;
Fermentation;
Furaldehyde;
analogs & derivatives;
chemistry;
Klebsiella oxytoca;
metabolism;
Lignin;
chemistry;
metabolism
- From:
Chinese Journal of Biotechnology
2013;29(3):350-357
- CountryChina
- Language:Chinese
-
Abstract:
To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.
