Improved production of microbial lipids in the two-liquid phase fermentation system.
- Author:
Riming YAN
1
;
Zuozuo AI
;
Ya WANG
;
Zhibin ZHANG
;
Qinggui ZENG
;
Zhu DU
Author Information
1. Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi, China.
- Publication Type:Journal Article
- MeSH:
Alkanes;
chemistry;
Biofuels;
Fermentation;
Industrial Microbiology;
methods;
Lipids;
biosynthesis;
Manihot;
metabolism;
Starch;
metabolism;
Trichosporon;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2013;29(4):536-539
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, we developed a two-liquid phase fermentation system by adding 1% n-dodecane as oxygen-vector to enhance the microbial lipids productivity of Trichosporon fermentans using cassava starch hydrolysate. Results suggest that the oxygen-vector could alleviate the oxygen shortage in flask fermentation. The cell mass and lipids concentration were 101.2 g/L and 50.28 respectively in 2 L fermenter with the presence of 1% n-dodecane. Additionally, gas chromatography analysis also reveals that the microbial lipids produced by T. fermentans contained a higher percentage of saturated fatty acid in the oxygen-vector case.
