Rapid detection of Listeria monocytogenes by immunomagnetic separation combined with selective medium.
- Author:
Yiming WEN
1
;
Zhiqing LI
;
Jiyu TONG
;
Junjian XIANG
Author Information
1. Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou 510632, Guangdong, China.
- Publication Type:Journal Article
- MeSH:
Bacteriological Techniques;
methods;
Culture Media;
chemistry;
Immunomagnetic Separation;
methods;
Listeria monocytogenes;
growth & development;
isolation & purification;
Sensitivity and Specificity
- From:
Chinese Journal of Biotechnology
2013;29(5):672-680
- CountryChina
- Language:Chinese
-
Abstract:
Listeria monocytogenes is a pathogenic bacterium, therefore, it is essential for food safety monitoring to establish a rapid and specific detecting method. In this study, immunomagnetic beads and selective medium were combined to detect Listeria monocytogenes at different concentrations (10(1)-10(5) CFU/mL). Other three types of Listeria spp., Staphylococcus aureus and Vibrio parahaemolyticus were also detected to conduct the cross-reaction analysis. Meanwhile, contaminated milk samples were prepared to explore the limit of detection of immunomagnetic beads combining with selective medium. Results showed that Listeria monocytogenes with the concentration of 10(3) CFU/mL and above was successfully detected. Milk samples were detected within 6 hours, with a detection limit of 0.7 CFU/mL. The method developed is capable of detecting milk samples within 30 h, which is 38 h faster compared with national standard method with the same sensitivity.
