Cost-effective production of protein by using cellulose-binding domain fusion tag in Corynebacterium glutamicum.
- Author:
Zhijing ZHAO
1
;
Huan JIANG
;
Wenting SHEN
;
Lianyan SONG
;
Guang HU
Author Information
1. Bioengineering Center, Shaoxing Institute of Technology, College of Engineering, Peking University, Shaoxing 312000, Zhejiang, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cellulases;
biosynthesis;
genetics;
Cellulose;
chemistry;
genetics;
Cloning, Molecular;
Corynebacterium glutamicum;
genetics;
metabolism;
Cost-Benefit Analysis;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
genetics;
metabolism;
Molecular Sequence Data;
Protein Engineering;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
Trichoderma;
genetics
- From:
Chinese Journal of Biotechnology
2013;29(5):691-694
- CountryChina
- Language:Chinese
-
Abstract:
The CBD gene from Trichoderma reesei was cloned into the Corynebacterium glutamicum secretion expression vector pXMJ19-sp, in which green fluorescent protein was inserted to obtain pXMJ19-sp-GFP-CBD. After induced by 0.5 mmol/L IPTG, GFP-CBD was expressed in Corynebacterium glutamicum at high level of 200 mg/L. The GFP-CBD could be purified to high purity with cellulose column. The results indicated CBD can be successfully used in Corynebacterium glutamicum expression system and thus offer an extremely simple, effective and scalable way for production of recombinant proteins.
