Response surface methodology to optimize marine microbe culture for producing fungi fibrinolytic compound.
- Author:
Tongwei SU
1
;
Bin BAO
;
Ting YAN
;
Chaoyan ZHANG
;
Yongshi BU
;
Wenhui WU
Author Information
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
- Publication Type:Journal Article
- MeSH:
Culture Techniques;
methods;
Fermentation;
Fibrinolytic Agents;
metabolism;
Seawater;
microbiology;
Stachybotrys;
growth & development;
metabolism;
Surface Properties
- From:
Chinese Journal of Biotechnology
2013;29(6):857-861
- CountryChina
- Language:Chinese
-
Abstract:
Response surface methodology was applied to optimize the fermentation conditions of FGFC1 (Fungi fibrinolytic compound 1). On the basis of single factor tests, response surface analysis was designed by Design-Expert, and the effects of culture time, ornithine hydrochloride addition and culture temperature on the yield of FGFC1 were studied, the predicted value and measured value were also contrasted. The results show the optimal culture conditions as follows: the culture time is 7 d, ornithine hydrochloride addition is 0.5% (M/V), culture temperature is 28 degrees C. Under these conditions, the yield of FGFC1 is 1 978.33 mg/L, which is consistent with the predicted value. It shows that the experiment is effective.
