Relationships among immune traits and MHC B-LBII genetic variation in three chicken breeds.
- Author:
Fuwei LI
1
;
Shuqing LI
;
Yan LU
;
Qiuxia LEI
;
Haixia HAN
;
Yan ZHOU
;
Bin WU
;
Dingguo CAO
Author Information
1. Institute of Poultry Science, Academy of Agricultural Sciences of Shandong Province, Jinan 250023, Shandong, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Breeding;
Chickens;
genetics;
immunology;
Major Histocompatibility Complex;
genetics;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide;
Polymorphism, Single-Stranded Conformational;
Sequence Analysis, DNA
- From:
Chinese Journal of Biotechnology
2013;29(7):904-913
- CountryChina
- Language:Chinese
-
Abstract:
We have assessed the relationships between immune trait (antibody titers of Sheep red blood cell, SRBC; Avian influenza, AI; Newcastle disease, ND) and varieties of MHC B-LBHII Gene in local chicken breeds (Wenshang Barred chicken, LH; Laiwu Black chicken, LWH; and Jining Bairi chicken, BR). We selected 300 chickens randomly from the three indigenous chicken populations. The variations of MHC B-L BII gene were detected by directly DNA sequencing and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The results indicated that there were about 19-22 nucleotide mutations in the three local breeds, which could affect 16-18 amino acid variations. Another results indicated that there was significantly relationship between seven to eight SNPs of the MHC B-LBII region and some immune traits (P < 0.05 or P < 0.01). Both locus G97A and locus T138A were found in the three species, which were significantly related to the antibodies of SRBC, ND and AI antibody titers (P < 0.05). Among them, the locus G97A was significantly associated with ND antibody titers (P < 0.05) in BR chicken, with SRBC antibody titers (P < 0.05) in LWH chicken, and with H9 antibody titers (P < 0.05) in LH chicken. Furthermore, locus T138A was significantly associated with H9 antibody titers in BR and LH chickens (P < 0.05). All those results suggest relationships among the different varieties of MHC B-LBII and immune traits in the three local breeds.
