Anti-tumor efficacy of P53 with 9R cell-penetrating peptides.
- Author:
Yuan LIU
1
;
Rui CHEN
;
Nan ZHANG
;
Xianlong YE
;
Yin BAI
;
Yuquan WEI
;
Guiping REN
;
Deshan LI
Author Information
1. Biopharmaceutical Teaching and Research Section, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell-Penetrating Peptides;
pharmacology;
Humans;
Tumor Suppressor Protein p53;
pharmacology
- From:
Chinese Journal of Biotechnology
2013;29(7):955-964
- CountryChina
- Language:Chinese
-
Abstract:
To enhance the penetration of P53 into tumor cells by fusion it with the cell penetrating peptide 9R. The fusion gene of 9R-p53 was cloned into the expression vector. The fusion protein, CPPs-P53, was expressed and purified. We detected the rate of cell growth inhibition and apoptosis by MTT and Annexin-V-FITC/PI double stained method respectively for measuring its effect on tumor cells. CPPs-P53 and P53 were successfully expressed and purified, the purity of both proteins reached up to 90%. MTT assay showed that the cell growth inhibition by CPPs-P53 was more efficient than P53, and the rate of cell growth inhibition is dose-dependent. The apoptosis experiment showed that P53 could induce apoptosis of tumor cells. Compared with the P53, CPPs-P53 had a more significant effect in inducing cell apoptosis (**P < 0.01). The CPPs-P53 shows more significant effects than P53 in inhibiting cell growth and inducing apoptosis on tumor cells.
