Effects of RNAi-mediated Gene Silencing of LRIG1 on Proliferation and Invasion of Glioma Cells
10.1007/s11596-012-0040-8
- Author:
MAO FENG
1
;
WANG BAOFENG
;
XI GUIFA
;
SUN WEI
;
ZHANG HUAQIU
;
YE FEI
;
GUO DONGSHENG
;
LEI TING
Author Information
1. Department of Neurosurgery and Sino-German Neuro-Oncology Molecular Laboratory,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
- Keywords:
glioma;
LRIG 1;
cell proliferation;
invasion
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(2):227-232
- CountryChina
- Language:Chinese
-
Abstract:
The effects of RNAi-mediated gene silencing of LRIG1 on proliferation and invasion of the human glioma cell line U251-MG and the possible mechanisms were explored in this study.The plasmids pGenesi12-LRIG1-shRNA1 and pGenesi12-LRIGl-shRNA2 were transfected into U251-MG glioma cells respectively by using Lipofectamine 2000 and the transfected cells in which the LRIGI expression was stably suppressed were selected by G418.The cells transfected with negative shRNA served as control.The expression levels of LRIG1 mRNA and protein were measured by qRT-PCR and Western blotting,respectively.The cell cycle was analyzed by flow cytometry.The results showed that LRIG1 mRNA expression was reduced by 70％ and 58％ and LRIG1 protein expression by 58％ and 26％ in U251-MG cells transfected with pGenesil2-LRIGl-shRNAl and pGenesil2-LRIG1-shRNA2 relative to the negative shRNA-transfected U251-MG cells.The proliferative capacity of the LRIG1 specific siRNA-transfected cells was stronger than that of control cells.Cell cycle analysis showed that silencing LRIG 1 significantly increased the percentage of S phase cells and the proliferation index (P＜0.01).Moreover,silencing LRIG1 could promote the invasion of U251-MG cells (P＜0.05).These findings suggested that LRIG1-targeting siRNA can exert a dramatically inhibitory effect on RNA transcription and protein expression of LRIG1,and LRIG1down-regulation could promote the proliferation of U251-MG cells,arrest U251-MG cells in S phase,and enhance the invasion of U251-MG cells.
