Inhibitory effects of microRNA-34a on cell migration and invasion of invasive urothelial bladder carcinoma by targeting Notch1.
10.1007/s11596-012-0065-z
- Author:
Chao ZHANG
1
;
Zhiyong YAO
;
Mingyang ZHU
;
Xin MA
;
Taoping SHI
;
Hongzhao LI
;
Baojun WANG
;
Jinzhi OUYANG
;
Xu ZHANG
Author Information
1. Department of Urology, Chinese PLA General Hospital, Beijing, China. enzo_zc@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Cell Movement;
genetics;
Down-Regulation;
genetics;
Female;
Gene Targeting;
Humans;
Male;
MicroRNAs;
genetics;
Middle Aged;
Neoplasm Invasiveness;
Receptor, Notch1;
physiology;
Transfection;
Tumor Cells, Cultured;
Urinary Bladder Neoplasms;
pathology;
physiopathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(3):375-382
- CountryChina
- Language:English
-
Abstract:
MicroRNAs (miRNAs or miRs) are a class of short, non-coding RNAs that participate in various oncological processes. This study aims to explore the roles of microRNA-34a (miR-34a) in invasive urothelial bladder carcinoma. miR-34a was transfected into bladder cancer cell lines 253J and J82. The miR-34a expression levels in tissues and cells were detected by using qRT-PCR. The Notch1 expression was detected by qRT-PCR and Western blotting. Cell migratory and invasive abilities were measured by Transwell chamber assay. Bioinformatics and luciferase assay were performed to predict and analyze the binding sites between miRNA-34a and Notch1. It was found that there was aberrant expression of miR-34a in bladder cancer tissues. Moreover, we revealed that ectopic expression of miR-34a suppressed cell migration and invasion, while forced expression of Notch1 increased cell migratory and invasive abilities. Finally, we observed that miR-34a transfection significantly down-regulated luciferase activity and reduced the mRNA and protein levels of Notch1. Our study concluded that microRNA-34a antagonizes Notch1 and inhibits cell migration and invasion of bladder cancer cells, which indicates the tumor-suppressive function of microRNA-34a in bladder cancer.
