TGF-β1-induced LPP expression dependant on Rho kinase during differentiation and migration of bone marrow-derived smooth muscle progenitor cells.
10.1007/s11596-012-0080-0
- Author:
Zhiling QU
1
;
Jun YU
;
Qiurong RUAN
Author Information
1. Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. zhilingqu70@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow;
metabolism;
physiology;
Cell Differentiation;
genetics;
Cell Movement;
genetics;
Cells, Cultured;
Cytoskeletal Proteins;
genetics;
metabolism;
LIM Domain Proteins;
genetics;
metabolism;
Mice;
Mice, Inbred C57BL;
Muscle, Smooth;
metabolism;
physiology;
Stem Cells;
metabolism;
physiology;
Transforming Growth Factor beta1;
genetics;
metabolism;
rho-Associated Kinases;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(4):459-465
- CountryChina
- Language:English
-
Abstract:
Lipoma preferred partner (LPP) has been identified as a protein which is highly selective for smooth muscle progenitor cells (SMPCs) and regulates differentiation and migration of SMPCs, but mechanisms of LPP expression are not elucidated clearly. The aim of the present study was to discuss the mechanisms by which LPP expression is regulated in the differentiation and migration of SMPCs induced by TGF-β1. It was found that TGF-β1 could significantly increase the expression of LPP, smooth muscle α-actin, smooth muscle myosin heavy chain (SM-MHC), and smoothelin in SMPCs. Moreover, inactivation of Rho kinase (ROK) with ROK inhibitors significantly inhibited LPP mRNA expression in TGF-β1-treated SMPCs and mouse aortic smooth muscle cells (MAoSMCs). At the same time, LPP silencing with short interfering RNA significantly decreased SMPCs migration. In conclusion, LPP appears to be a ROK-dependant SMPCs differentiation marker that plays a role in regulating SMPCs migration.
