Metabonomic phenotype of "formula corresponding to pattern types" based on "qi and yin deficiency pattern" of myocardial ischemia rat model.
- Author:
Bei YAN
1
;
Ji-Ye A
;
Hai-Ping HAO
;
Guang-Ji WANG
;
Lin-Sheng LIU
;
Wei-Bin ZHA
;
Ying ZHANG
;
Sheng-Hua GU
Author Information
1. Department of Clinical Pharmacology, Beijing Hospital, Ministry of Health, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Arrhythmia Agents;
pharmacology;
Drug Combinations;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
pharmacology;
Endothelin-1;
blood;
Gas Chromatography-Mass Spectrometry;
methods;
Injections;
Male;
Medicine, Chinese Traditional;
Metabolomics;
methods;
Myocardial Ischemia;
blood;
metabolism;
pathology;
Panax;
chemistry;
Plants, Medicinal;
chemistry;
Propranolol;
pharmacology;
Qi;
Rats;
Rats, Sprague-Dawley;
Salvia;
chemistry;
Schisandraceae;
chemistry;
Yin Deficiency;
metabolism
- From:
Acta Pharmaceutica Sinica
2011;46(8):976-982
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the scientific connotation of "Fangzhengduiying (formula corresponding to pattern types)", "Qiyinliangxuzheng (Qi and Yin deficiency pattern)" of myocardial ischemia rat model and GC-TOF/MS based metabonomic method were used for comparing the effects of Sheng-mai injection, Salvia injection and propranolol in the present study. After data processing and pattern recognition, Sheng-mai injection showed better efficacy than the other two drugs in accordance with not only visual observation from PLS-DA scores plots but also the number of abnormal endogenous compounds restored to the normal level. Further studies showed that Sheng-mai injection could normalize the level of plasma endothelin-1, the index related to cardiovascular diseases and sleep disorders, which verified the results of metabonomics. Finally, the regulated metabolites and related metabolic pathways were analyzed, and it was supposed that the effects of Sheng-mai injection involved in the alternation of energy metabolism, lipid metabolism, amino acids metabolism, and so on. These findings provided scientific evidence to Shengmai "Fang" used for "Qi and Yin deficiency pattern" correspondingly, indicating that metabonomics has great potential in traditional Chinese medical research, which provides a novel approach and way to modernization of traditional Chinese medicine.
