Pharmacokinetics of cantide, an antisense oligonucleotide, and its metabolites in rhesus monkeys.
- Author:
Xiu-zhong WANG
1
;
Shi-hong WANG
;
Hai-feng SONG
;
Qing-qing WANG
;
Sheng-qi WANG
Author Information
1. The 188th Hospital of PLA, Chaozhou 521000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Area Under Curve;
Electrophoresis, Capillary;
methods;
Female;
Half-Life;
Infusions, Intravenous;
Macaca mulatta;
Male;
Oligonucleotides, Antisense;
blood;
metabolism;
pharmacokinetics;
Phosphorothioate Oligonucleotides;
blood;
metabolism;
pharmacokinetics;
Solid Phase Extraction
- From:
Acta Pharmaceutica Sinica
2011;46(11):1370-1373
- CountryChina
- Language:Chinese
-
Abstract:
To study the pharmacokinetics of cantide, an antisense oligonucleotide, and its metabolites after iv gtt administration in rhesus monkeys, a dual solid phase extraction pretreatment method coupling with non-gel sieving capillary electrophoresis analysis method was used for determination of cantide and its metabolites in plasma and their pharmacokinetic parameters were calculated. The pharmacokinetic behavior of cantide and its metabolites (M1 and M2) after iv gtt administration (8, 16 and 24 mg kg(-1)) in rhesus monkeys were investigated. After iv gtt administration of cantide to rhesus monkeys, cantide in plasma was eliminated rapidly and the terminal elimination half-life (t1/2) was 57.91-77.97 min, the correlation coefficients (r) to the dose of Cmax AUC(o-inf) and AUC(0-t) of the prototype was 0.9918, 0.9568 and 0.9773, respectively. The metabolites of cantide reached the Cmax following cantide immediately and the Cmax of metabolites were lower than that of the prototype. The CL(S) of cantide and its metabolites (M1 and M2) were 1.60-2.19, 5.92-8.58 and 6.07-8.78 mL min(-1) kg(-1), respectively. So, it is concluded that the Cmax of cantide and its metabolites increased with the dose, which is the same as their AUC(0-inf) and AUC(0-t). The CL(S) of metabolites were higher than that of the prototype. The MRT and t1/2 of metabolites in the high dose group increased obviously.
