Identification of the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration.
- Author:
Lin-ling LU
1
;
Yan SHU
;
Da-wei QIAN
;
Shu-lan SU
;
Jin-ao DUAN
;
Ye-fei QIAN
;
Cai-fu XUE
Author Information
1. Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
metabolism;
pharmacokinetics;
Flavanones;
analysis;
metabolism;
pharmacokinetics;
Glucosides;
analysis;
metabolism;
pharmacokinetics;
Glycyrrhizic Acid;
analysis;
metabolism;
pharmacokinetics;
Hesperidin;
analogs & derivatives;
analysis;
metabolism;
pharmacokinetics;
Hydroxylation;
Male;
Plants, Medicinal;
chemistry;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:
Acta Pharmaceutica Sinica
2011;46(11):1374-1379
- CountryChina
- Language:Chinese
-
Abstract:
Sinisan is a widely used traditional Chinese medicine (TCM) in treating various diseases; however, the in vivo metabolic profile of its multiple components remains unknown. In this paper, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was applied to identify the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration. Using MS(E) and mass defect filter techniques, 41 metabolites of 10 parent compounds (naringin, naringenin, hesperidin, neohesperidin, liquiritin, liquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, saikosaponin a and saikosaponin d) were detected and tentatively identified. It was shown by our results that these compounds was metabolized to the forms of hydroxylation, glucuronidation, sulfation, glucuronidation with sulfation and glucuronidation with hydroxylation in vivo.
