Effects of ligustrazine on hippocampal dentate gyrus cell proliferation after focal cerebral ischemia in adult rats.
- Author:
Fen QIU
1
;
Yong LIU
;
Peng-bo ZHANG
;
Ying-fang TIAN
;
Cun-fang QI
;
Jian-jun ZHAO
;
Jie YANG
;
Xin-li XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Dentate Gyrus; drug effects; pathology; Infarction, Middle Cerebral Artery; physiopathology; Injections, Intraperitoneal; Male; Pyrazines; administration & dosage; pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley; Time Factors
- From: Journal of Southern Medical University 2006;26(10):1400-1403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of ligustrazine on cell proliferation in hippocampal dentate gyrus subgranular zone (SGZ) after focal cerebral ischemia in adult rats.
METHODSMiddle cerebral artery occlusion (MCAO) model was established in adult rats by placement of an intraluminal filament at the origin of the MCA. Ligustrazine was administered intraperitoneally at a daily dose of 80 mg/kg starting at 2 h after MCAO, and BrdU (50 mg/kg daily) was also injected intraperitoneally starting at 4 h after MCAO. BrdU-positive cells in the SGZ were counted 7, 14 and 24 days after MCAO, respectively.
RESULTSCompared with sham operation group, ischemic ipsilateral BrdU-positive cells in the ischemic model group increased 7 days after MCAO, reaching the peak on day 14, and decreased on day 21 (P<0.01). The number of ischemic ipsilateral BrdU-positive cells in ligustrazine group was significantly greater than that in the ischemic model group on days 7, 14 and 21 (P<0.01), and maintained the high level on day 21.
CONCLUSIONLigustrazine possesses long lasting effect of promoting cell proliferation in the SGZ after focal cerebral ischemia in adult rats.
