Association of abnormal cyclooxygenase-2 gene expression with colorectal carcinoma metastasis.
- Author:
Zu-guo LI
1
;
Teng-fei LIU
;
Wei-bing XIE
;
Jun ZHOU
;
Li YU
;
Yan-qing DING
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Colorectal Neoplasms; enzymology; pathology; Cyclooxygenase 2; biosynthesis; genetics; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Lymphatic Metastasis; RNA, Messenger; biosynthesis; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2006;26(10):1408-1411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.
METHODSSP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.
RESULTSSW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.
CONCLUSIONElevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.
