Construction and identification of tetracycline-inducible human hepatocyte growth factor eukaryotic expression vector.
- Author:
Shu-Ting REN
1
;
Lin-hua YU
;
Chang-fu XU
;
Guang-dao GAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Complementary; genetics; Eukaryotic Cells; cytology; metabolism; Gene Expression; drug effects; Genetic Vectors; genetics; Hepatocyte Growth Factor; biosynthesis; genetics; Humans; Tetracycline; pharmacology
- From: Journal of Southern Medical University 2006;26(10):1443-1445
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a tetracycline-inducible eukaryotic expression vector containing human hepatocyte growth factor (HGF) cDNA.
METHODSHuman HGF cDNA fragment was obtained by PCR from pUC-SRalpha/HGF plasmid and inserted into the restriction site between Mlu I and Sal I of the tetracycline-inducible eukaryotic expression vector pBI-L. pBI-L-HGF was constructed by DNA recombination in vitro, and was identified by restriction endonucleases digestion and sequencing.
RESULTSThe fragment of pBI-L-HGF digested with restriction endonucleases well corresponded to expectation, and the sequence of inserted HGF cDNA was correct according to the GenBank.
CONCLUSIONThe tetracycline-inducible eukaryotic expression vector of human HGF pBI-L-HGF has been constructed successfully, which allows further study of HGF gene therapy with much safety and easy manipulation.
