ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei).
- Author:
Hui XIE
1
;
Zhi CHAO
;
Ke-ke HUO
;
Bing-yi WU
;
Sheng-li PAN
Author Information
- Publication Type:Journal Article
- MeSH: Bupleurum; classification; genetics; DNA, Plant; chemistry; genetics; DNA, Ribosomal Spacer; chemistry; genetics; Molecular Sequence Data; Phylogeny; Plant Roots; genetics; Sequence Analysis, DNA; Species Specificity
- From: Journal of Southern Medical University 2006;26(10):1460-1463
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs.
METHODThe ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2.
RESULTSHomologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%.
CONCLUSIONThe ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri.
