Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection.
- Author:
Ying-ying FAN
1
;
Li-xian XU
;
Ai-dong WEN
;
Hui ZHANG
;
Chun-ran LIU
;
Wei LI
;
Xiao-peng MEI
Author Information
- Publication Type:Journal Article
- MeSH: Anesthetics, Intravenous; blood; chemistry; Chromatography, High Pressure Liquid; methods; Fluorescence; Humans; Propofol; blood; chemistry; Reproducibility of Results; Spectrometry, Fluorescence; methods
- From: Journal of Southern Medical University 2006;26(10):1510-1512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum.
METHODSHuman serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (90:10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm.
RESULTSThe calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%.
CONCLUSIONSThe method is accurate, sensitive and simple for propofol determination in clinical anesthesia.
