Establishment of miniCTTA multiplex set with fluorescence-labeled primers.
- Author:
Chao LIU
1
;
Hui-pin WANG
;
Hong-yu SUN
;
Hui-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amelogenin; genetics; Base Sequence; DNA; analysis; isolation & purification; DNA Fingerprinting; DNA Primers; Genetic Markers; genetics; Humans; In Situ Hybridization, Fluorescence; Microsatellite Repeats; genetics; Nucleic Acid Amplification Techniques; methods; Reproducibility of Results
- From: Journal of Southern Medical University 2006;26(11):1574-1576
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.
METHODSThe length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.
RESULTSThe genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.
CONCLUSIONMiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.
