Isolation and in vitro culture of follicular papilla cells from rat vibrissae.
- Author:
Xi-jin SUN
1
;
Zhi-qi HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Culture Techniques; Cell Proliferation; Cell Separation; methods; Cells, Cultured; Hair Follicle; cytology; metabolism; Rats; Rats, Wistar; Vibrissae; cytology; metabolism
- From: Journal of Southern Medical University 2006;26(11):1619-1620
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple method for isolating and culturing follicular papilla cells from rat vibrissae.
METHODSThe intact follicles were obtained and digested in 0.2% collagenase I with agitation on a rotary stirrer at 100 r/min at 37 degrees C; for 3 h. The suspension was centrifuged at 300 r/min and the papilla cells were collected and suspended in DMEM for cell culture. The adhesion efficiency of the dermal papilla cells was evaluated and compared with that of the cells obtained by microdissection.
RESULTS AND CONCLUSIONThe described procedure allowed efficient and rapid isolation of the dermal papilla cells from rat vibrissae and ensured improved adhesion of the dermal papillae and outgrowth of the cells with reduced labor and risk of contamination. The cells obtained with this procedure were positive for alpha-smooth muscle actin staining.
