Antisense oligonucleotide against survivin induces apoptosis and enhances adriamycin sensitivity of SMMC-7721/ADM cells.
- Author:
Peng GAO
1
;
Chi-hua FANG
;
Gang-qing ZHANG
;
Zheng YAN
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; Apoptosis; drug effects; genetics; physiology; Blotting, Western; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; Flow Cytometry; Humans; Inhibitor of Apoptosis Proteins; Liposomes; chemistry; Liver Neoplasms; genetics; metabolism; pathology; Microtubule-Associated Proteins; genetics; metabolism; Neoplasm Proteins; genetics; metabolism; Oligonucleotides, Antisense; chemistry; genetics; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection; methods
- From: Journal of Southern Medical University 2006;26(11):1644-1647
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of survivin-specific antisense oligonucleotide (ASODN) delivered via liposome on the growth and apoptosis of drug-resistant human hepatic cancer cell line SMMC-7721/ADM and the sensitivity of the cells to adriamycin (ADM).
METHODSSMMC-7721/ADM cells were divided into 6 groups and treated with liposome, ADM, sense oligonucleotide (SODN), SODN+ADM, 400 ng/ml ASODN, and 400 ng/ml ASODN+ADM group, respectively. MTT assay was used to calculate the relative survival rates of the cells, and the changes in cell apoptosis and cycle were detected with flow cytometry. RT-PCR and Western blotting were performed to detect the expressions of survivin mRNA and protein, respectively.
RESULTSThe apoptotic rate of ASODN-treated cells was much higher than that of the control cells. survivin protein expression showed no significant variation between cells treated with liposome, ADM, SODN, and SODN+ADM (P>0.05), whereas compared with these 4 groups, cells treated with 400 ng/ml ASODN and 400 ng/ml ASODN+ADM had significantly lowered survivin mRNA expression (P<0.05), without significant differences between the latter two groups (P>0.05). SMMC-7721/ADM cells cultured in the presence of ASODN and adriamycin showed significant growth inhibition in comparison with ASODN group and ADM group.
CONCLUSIONsurvivin-specific ASODN can enhance the sensitivity of SMMC-7721/ADM cells to ADM by depressing survivin expression in the cells, thus improves the effect of ADM chemotherapy for liver cancer.
