Sampling of exhaled gas after intravenous administration of octafluoropropane-containing human albumin micropheres in dogs.
- Author:
Ling YANG
1
;
Zhi-liang CHEN
;
Shi-ting LIU
;
Li YANG
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; administration & dosage; analysis; pharmacokinetics; Animals; Dogs; Exhalation; Female; Fluorocarbons; administration & dosage; analysis; pharmacokinetics; Gas Chromatography-Mass Spectrometry; methods; Humans; Injections, Intravenous; Male; Microspheres; Reproducibility of Results
- From: Journal of Southern Medical University 2006;26(12):1706-1708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for quantitative collection of exhaled gas in anesthetized dogs at given time following intravenous administration of octafluoropropane (OFP)-containing human albumin micropheres for assessing the gas kinetics of OFP.
METHODSOFP-containing albumin micropheres were administered intravenously at 0.4, 0.8 and 1.2 ml/kg, respectively, in anesthetized and ventilated dogs. The exhaled air samples were analyzed by gas chromatography-tandem mass spectrometry (GC-MS-MS).
RESULTSThe correlation curve between the area under curve (AUC) and administered dose was roughly linear (Y=1162.5X-417.38, r square=0.949 9). The total recovery rate was (119.49-/+27.62)% which was not significantly different from the rate of 100% (P>0.05). GC-MS-MS was accurate, sensitive, precise and applicable for OFP determination.
CONCLUSIONThe sampling method is useful for characterizing OFP pharmacokinetics in dogs, and also applicable for studying the pharmacokinetics of other gas-containing drugs.
