Expression of Smo protein and the downstream transcription factor Gli1 protein in Sonic hedgehog signal transduction pathway in gastric carcinoma.
- Author:
Zhen-xiang RONG
1
;
Chi-hua FANG
;
Da-jian ZHU
;
Sheng-jun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; physiopathology; Adult; Aged; Female; Hedgehog Proteins; physiology; Humans; Immunohistochemistry; Male; Middle Aged; Receptors, G-Protein-Coupled; biosynthesis; Signal Transduction; Smoothened Receptor; Stomach Neoplasms; metabolism; pathology; physiopathology; Transcription Factors; biosynthesis; Zinc Finger Protein GLI1
- From: Journal of Southern Medical University 2006;26(12):1728-1731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of Smo protein and the downstream transcription factor Gli1 protein in Sonic hedgehog signal transduction pathway in gastric carcinoma.
METHODSA tissue microarray was constructed using 85 gastric carcinoma and 25 normal gastric mucosa specimens. The expression of Smo and Gli1 proteins were detected immunohistochemically and the correlation between their expression in gastric carcinoma was analyzed.
RESULTSOnly weak expression, if any, of Smo and Gli1 proteins was detected in normal gastric mucosa, but in papillary adenocarcinoma, tubular adenocarcinoma and poorly differentiated adenocarcinoma, their expressions were significant increased as the differentiation degree was lowered. Smo protein expression in gastric carcinoma was significantly correlated with that of Gli1 protein with correlation coefficient of 0.989 (P<0.001).
CONCLUSIONThe abnormal activity of Sonic hedgehog signal transduction pathway may play an important role in the occurrence of papillary adenocarcinoma, tubular adenocarcinoma and poorly differentiated adenocarcinoma, and this abnormality is associated with Smo protein overexpression, which upregulates the expression of the downstream transcription factor Gli1 protein.
