Isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells.
- Author:
Jun LIU
1
;
Jing AN
;
Jin-quan CEHN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; physiology; Cell Separation; methods; Cell Transdifferentiation; drug effects; physiology; Cells, Cultured; Epidermal Growth Factor; pharmacology; Female; Fetus; Fibroblast Growth Factor 2; pharmacology; Hepatocytes; cytology; metabolism; Insulin; pharmacology; Intermediate Filament Proteins; biosynthesis; Islets of Langerhans; cytology; drug effects; Leukemia Inhibitory Factor; pharmacology; Liver; Male; Nerve Tissue Proteins; biosynthesis; Nestin; Rats; Rats, Wistar; Stem Cell Factor; pharmacology
- From: Journal of Southern Medical University 2006;26(12):1757-1759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method for isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells.
METHODSHepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25 ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.
RESULT AND CONCLUSIONNestin-positive cells were obtained from fetal rat liver, which can differentiate into islet beta cells after culture and expansion in vitro.
