Inhibitory effect of Biejiajianwan pills on tumor growth and proliferating cell nuclear antigen expression in mice bearing H22 cell-induced tumor.
- Author:
Xu-hui ZHANG
1
;
Da-li CHEN
;
Rong-cheng LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Phytogenic; pharmacology; therapeutic use; Carcinoma, Hepatocellular; metabolism; pathology; prevention & control; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; therapeutic use; Immunohistochemistry; Liver Neoplasms, Experimental; metabolism; pathology; prevention & control; Male; Mice; Proliferating Cell Nuclear Antigen; biosynthesis; Random Allocation; Tumor Burden; drug effects
- From: Journal of Southern Medical University 2006;26(12):1791-1793
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-tumor effect of Biejiajianwan pills and explore its mechanism.
METHODSMice bearing neoplasm induced by H22 cell inoculation were randomized into 4 groups, namely the negative control group, positive control group (with cyclophosphamide treatment), and high- and low-dose Biejiajianwan pill group. The tumor-bearing mice received corresponding treatments for 15 consecutive days, after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. The tumor mass was also weighed and the tumor inhibition rate calculated. Immunohistochemistry was performed to evaluate the expression intensity of proliferating cell nuclear antigen (PCNA) in the tumor tissue.
RESULTSThe pills significantly inhibited the growth of the implanted tumor and reduced the expression of PCNA in the tumor.
CONCLUSIONOne of the possible mechanisms of the anti-tumor effects of Biejiajian pills might be associated with the inhibition of PCNA expression in the tumor.
