Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease.
- Author:
Yongchang DIWU
1
;
Jinzhou TIAN
;
Jing SHI
Author Information
- Publication Type:Journal Article
- MeSH: Acetylglucosaminidase; metabolism; Alzheimer Disease; chemically induced; enzymology; metabolism; Animals; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; pharmacology; Glycosylation; Hippocampus; enzymology; metabolism; Male; N-Acetylglucosaminyltransferases; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Streptozocin; tau Proteins; metabolism
- From: Journal of Southern Medical University 2013;33(10):1442-1447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Xixin decoction (XXD) on O-GlcNAc transferase (OGT) and O-GlcNAc glycosidase in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease (SAD) and explore the possible mechanism.
METHODSMale SD rats were randomly divided into sham-operated group, model group, donepezil group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting.
RESULTSXXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of O-GlcNAc glycosidase (P<0.05 or 0.01). OGT and O-GlcNAc glycosidase expressions showed no significant differences between the model group and donepezil group (P>0.05).
CONCLUSIONXXD can regulate the expression of OGT and O-GlcNAc glycosidase to enhance O-GlcNAc glycosylation of tau proteins in the hippocampus of SAD rats.
