Effects of peroxisome proliferator-activated receptors γ on the expression of insulin receptor substrate-4 gene in rat cortical neurons and mouse brain.
- Author:
Hongyan ZHANG
1
;
Siying MENG
;
Lifang LIN
;
Qiaoqi WU
;
Riyang ZHOU
;
Xuemin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Cells, Cultured; Cerebral Cortex; cytology; metabolism; Female; Gene Transfer Techniques; Insulin Receptor Substrate Proteins; genetics; metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurons; cytology; metabolism; PPAR gamma; agonists; genetics; metabolism; Pregnancy; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Thiazolidinediones; pharmacology; Up-Regulation
- From: Journal of Southern Medical University 2013;33(10):1463-1466
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on insulin receptor substrate-4 (IRS-4) gene expression in the brain.
METHODSPrimarily cultured cortical neurons from E17-18 Sprague Dawley rats, after 1 week of plating, were exposed to 10 µmol/L PPARγ agonist rosiglitazone for 0, 1, 4 or 24 h. Adult C57BL/6J mice or conditional brain PPARγ knock-out mice (B-PPARγ-KO, BKO) received an intraperitoneal injection of rosiglitazone in 10% DMSO at 12 mg/kg or injection of the same volume of saline containing 10% DMSO. The effect of rosiglitazone on the survival of the neurons was examined by MTT assay. The expression of IRS-4 mRNA was analyzed by real-time quantitative PCR.
RESULTSThe survival of the cortical neurons showed no significant difference between the agonist groups and the control group. The expression of IRS-4 mRNA was significantly up-regulated in the cortical tissues and neurons of the agonist groups compared with the control groups (P<0.05), but in BKO mice without treatment, IRS-4 mRNA expression was significantly down-regulated (P<0.05).
CONCLUSIONPPARγ can enhance the expression of IRS-4 mRNA in the brain.
