Changes of fecal flora and its correlation with inflammatory indicators in patients with inflammatory bowel disease.
- Author:
Ting ZHANG
1
;
Ye CHEN
;
Zhongqiu WANG
;
Youlian ZHOU
;
Shaoheng ZHANG
;
Pu WANG
;
Shan XIE
;
Bo JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bacteria; isolation & purification; Bacteroides; isolation & purification; Bifidobacterium; isolation & purification; Biomarkers; analysis; Clostridium; isolation & purification; Colitis, Ulcerative; microbiology; Crohn Disease; microbiology; Enterococcus; isolation & purification; Eubacterium; isolation & purification; Feces; microbiology; Female; Humans; Inflammatory Bowel Diseases; etiology; microbiology; Lactobacillus; isolation & purification; Male; Peptococcus; isolation & purification; Saccharomyces; isolation & purification
- From: Journal of Southern Medical University 2013;33(10):1474-1477
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in fecal flora and its correlation with the occurrence and progression of inflammatory bowel disease (IBD).
METHODSWe collected fresh fecal specimens from 167 IBD patients (including 113 with ulcerative colitis and 54 with Crohn's disease) and 54 healthy volunteers. The fecal flora was analyzed by gradient dilution method and the data of inflammatory markers including WBC, PLT, CRP and ESR were collected to assess the association between the fecal flora and the inflammatory markers.
RESULTSThe species Enterrococcus (6.60∓0.23, P<0.01), Saccharomyces (2.22∓0.27, P<0.05), Bacteriodes (5.57∓0.28, P<0.001), Bifidobacterium (5.08∓0.30, P<0.01), Peptococcus (6.22∓0.25, P<0.001), Lactobacillus (6.00∓0.26, P<0.001), and Clostridium (3.57∓0.30, P<0.05) all increased significantly, while Eubacterium (1.56∓0.24, P<0.01) reduced markedly in patients with ulcerative colitis compared with those in the control subjects. Enterrococcus (6.93∓0.28, P<0.01), Saccharomyces (2.73∓0.37, P<0.01), Bacteriodes (4.32∓0.52, P<0.05), Bifidobacterium (4.88∓0.42, P<0.05), Peptococcus (6.19∓0.32, P<0.01) and Lactobacillus (4.73∓0.47, P<0.001) all increased significantly and Eubacterium (1.01∓0.29, P<0.01) and Clostridium (0.87∓0.31, P<0.01) decreased in patients with Crohn's disease. The positivity rates of bacterial culture were consistent with the results of quantitative analysis of the fecal flora. The changes in fecal flora did not show a significant correlation with these inflammatory markers.
CONCLUSIONIBD patients have fecal flora imbalance compared with the healthy controls, and this imbalance may contribute to the occurrence and progression of IBD. The decline of Eubacterium contributes to the occurrence and development of IBD.
