Human stem cells from apical papilla can regenerate dentin-pulp complex.
- Author:
Huacui XIONG
1
;
Ke CHEN
;
Yibin HUANG
;
Caiqi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alkaline Phosphatase; analysis; Animals; Cell Culture Techniques; Cell Differentiation; Coculture Techniques; Dental Papilla; cytology; Dental Pulp; cytology; Extracellular Matrix Proteins; analysis; Female; Humans; Integrin-Binding Sialoprotein; analysis; Mice; Mice, Nude; Odontogenesis; physiology; Osteocalcin; analysis; Phosphoproteins; analysis; Sialoglycoproteins; analysis; Stem Cells; chemistry; physiology; Tissue Engineering; methods; Young Adult
- From: Journal of Southern Medical University 2013;33(10):1512-1516
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo regenerate dentin-pulp complex by tissue engineering with human stem cells from apical papilla cells (SCAP) as the seed cells.
METHODSSCAP was separated from from normal human impacted third molars with immature roots by outgrowth culture. The cells were then cultured in the differentiation medium for 3 weeks or in normal medium for 60 days, and analyzed for mineralization potential by Alizarin red staining. The osteo/odontogenic markers including alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OC) and dentin sialoprotein (DSP) were investigated by immunofluorescence staining and reverse transcription-polymerase chain reaction. The co-cultured mixture of SCAP and HA/TCP, or HA/TCP alone was implanted subcutaneously on the back of nude mice for 8 weeks, and the implants were collected and examined by HE and immunohistochemical staining.
RESULTSRound alizarin red-positive nodules formed in the isolated cells after cell culture in the differentiation medium for 3 weeks or in normal medium for 60 days with positive staining for osteo/odontogenic markers. SCAP with HA/TCP could regenerate pulp-dentin complex-like tissue in nude mice. The cells near the dentin-like tissue were positive for DSP. No mineral tissue was found in mice receiving HA/TCP implantation.
CONCLUSIONSSCAP may serve as a promising seed cell for dentin-pulp complex tissue engineering.
