Pulmonary expression of Nr1d1 in rats with ventilation-induced lung injury.
- Author:
Jiaqi HU
1
;
Chunxiao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Circadian Rhythm; Down-Regulation; Female; Lung; metabolism; Male; Nuclear Receptor Subfamily 1, Group D, Member 1; metabolism; Oligonucleotide Array Sequence Analysis; Random Allocation; Rats; Rats, Sprague-Dawley; Respiration, Artificial; adverse effects; methods; Reverse Transcriptase Polymerase Chain Reaction; Tidal Volume; Ventilator-Induced Lung Injury; metabolism
- From: Journal of Southern Medical University 2013;33(10):1544-1546
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in the expression of nuclear receptor subfamily1 (Nr1d1) in the lungs of rats with ventilation-induced lung injury and explore the molecular mechanism.
METHODSSeventy-two rats were randomly divided into 4 groups for observation at 4 time points (1:00 AM, 7:00 AM, 1:00 PM and 7:00 PM), and each group was further divided into 3 subgroups (Group FB, Group LV and Group HV). After ventilation for 3 h, the rats were sacrificed and lung tissue samples were obtained for testing the expression of Nr1d1 using gene chip technology and RT-PCR.
RESULTSNr1d1 gene expression was down-regulated in HV group as shown by gene chip RT-PCR data. Cosine analysis showed that Nr1d1 expression exhibited a rhythmicity in FB group (P<0.05).
CONCLUSIONThe rhythmicity of Nr1d1 expression disappears as the tidal volume the increase. Diminished Nr1d1 expression is closely correlated with ventilation-induced lung injury in rats.
