Effects of Toll-like receptors on indoleamine 2, 3-dioxygenase mRNA levels in human trophoblast HTR-8/SVneo cells.
- Author:
Wei XU
1
;
Guibo YANG
;
Jiazhong DUAN
;
Yue WANG
;
Wenrong YAO
;
Xueqing LIU
;
Xuemei CHEN
;
Yubin DING
;
Yingxiong WANG
;
Junlin HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Female; Humans; Indoleamine-Pyrrole 2,3,-Dioxygenase; genetics; metabolism; Interferon-gamma; pharmacology; Ligands; Poly I-C; pharmacology; RNA, Messenger; metabolism; Toll-Like Receptors; genetics; metabolism; Trophoblasts; cytology; metabolism
- From: Journal of Southern Medical University 2013;33(11):1559-1564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of Toll-like receptors (TLRs) mRNA in human trophoblast HTR-8/SVneo cells and the changes in indoleamine 2,3-dioxygenase (IDO) mRNA expression in response to TLR ligand stimulation.
METHODSThe expressions of TLRs and IDO mRNA in human HTR-8/SVneo cells were tested by RT-PCR, and the changes in IDO mRNA levels after exposure to TLR3, TLR4, TLR7/8, and TLR9 ligands were quantitatively analyzed with real-time PCR.
RESULTSIDO and TLR1-10 mRNAs were expressed in HTR-8/SVneo cells. As the cell culture time extended, IDO mRNA expression level tended to increase within 48 h. After stimulation with the TLR ligands, the expression of TLR-3 mRNA was down-regulated while the expression of TLR-4, 7, 8, and 9 mRNA up-regulated. Stimulation of the cells with poly(I:C) lowered the expression of IDO mRNA while IFN-γ increased its expression.
CONCLUSIONSThe expression of IDO mRNA is associated with the nutrition of the maternal-fetal interface. Stimulation with the TLR ligands affects the expression of IDO and TLR mRNA expressions in the cells, which verifies the functional activity of TLRs and suggests a role of IDO in TLR pathway-dependent antiviral immunity.
