Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB.
- Author:
Yi XIAO
1
;
Kun GAO
;
Yong YANG
;
Jinqi YAN
;
Liang ZHANG
;
Yu WANG
;
Yuanji XU
;
Renli TIAN
;
Zhiyan DU
;
Jiyun YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibody Formation; Antibody Specificity; Antigens, Neoplasm; genetics; immunology; Electroporation; Gene Fusion; Granulocyte-Macrophage Colony-Stimulating Factor; genetics; immunology; HEK293 Cells; Humans; Injections, Intramuscular; Male; Mice; Mice, Inbred BALB C; Plasmids; Random Allocation; Recombinant Fusion Proteins; genetics; immunology; Transfection; Vaccines, DNA; genetics; immunology
- From: Journal of Southern Medical University 2013;33(11):1628-1631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX- tG250FcGB.
METHODSThe DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared.
RESULTSThe vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection.
CONCLUSIONElectroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.
