Absorption, distribution and elimination of (59)Fe- corn polysaccharide iron complex in rats: a study with radioactivity isotope tracing.
- Author:
Yuyan ZHOU
1
;
Jianmin LIAO
;
Zilong SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Area Under Curve; Coordination Complexes; administration & dosage; pharmacokinetics; urine; Feces; chemistry; Female; Intestinal Absorption; Iron; administration & dosage; pharmacokinetics; urine; Iron Radioisotopes; Male; Polysaccharides; administration & dosage; isolation & purification; pharmacokinetics; urine; Random Allocation; Rats; Rats, Sprague-Dawley; Tissue Distribution; Zea mays; chemistry
- From: Journal of Southern Medical University 2013;33(11):1638-1642
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for detecting plasma concentration of corn polysaccharide iron complex (CPIC) and investigate its absorption, distribution and elimination in rats.
METHODSUsing radioactivity isotope tracing method, we detected the radioactivity of (59)Fe-CPIC in the plasma of rats at different time points by gavages of 3 doses (28.0, 14.0, and 7.0 mg/kg) (59)Fe-CPIC in SD rats. The pharmacokinetic parameters was obtained using DAS 2.0 program for analysis of tissue distribution and elimination of (59)Fe-CPIC.
RESULTSThe standard curve was linear within the range of 0.14-141 µg/ml (r=0.9999, n=5). The average recovery was 95% with a relative standard deviation no more than 15%. The pharmacokinetic parameters at 3 doses obtained, namely t1/2 and AUC (0-), were 214∓104, 231∓110, and 181∓81 min, and 1986.3∓513.3, 737.0∓467.0, and 315.1∓226.1 mg·min-1·L(-)1, respectively. (59)Fe-CPIC were detected in all the 13 tissues types examined and high radioactivity intensity was found in the gastrointestinal tract, hematogenic organs and other organs rich in blood. (59)Fe-CPIC was eliminated after intragastric administration primarily via the feces in rats.
CONCLUSIONThe method we established is easy and specific, and the pharmacokinetic parameters of (59)Fe-CPIC fit the two- compartment open model.
