Effect of piperphentonamine hydrochloride on expressions of interleukin-1β and TNF-α mRNA and Fas protein in guinea pigs with cochlear ischemia/reperfusion injury.
- Author:
Yonghe LI
1
;
Wei LI
;
Jian WU
;
Hao CHEN
;
Liangcai WAN
Author Information
- Publication Type:Journal Article
- MeSH: 3,4-Methylenedioxyamphetamine; analogs & derivatives; pharmacology; Animals; Cochlea; blood supply; metabolism; pathology; Female; Guinea Pigs; Interleukin-1beta; genetics; metabolism; Male; Neuroprotective Agents; pharmacology; Organ of Corti; metabolism; RNA, Messenger; metabolism; Random Allocation; Reperfusion Injury; metabolism; Spiral Ganglion; metabolism; Stria Vascularis; metabolism; Tumor Necrosis Factor-alpha; genetics; metabolism; fas Receptor; metabolism
- From: Journal of Southern Medical University 2013;33(11):1669-1672
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between IL-1β and TNF-α mRNA and Fas protein expressions and cochlear ischemia reperfusion injury and investigate the protective mechanism of PPTA against cochlear reperfusion injury.
METHODSSixty-four guinea pigs were randomly divided into normal control group, blank control group, ischemia/reperfusion (by clamping the bilateral vertebral artery and right common carotid artery for 1 h) control group, and ischemia/reperfusion with PPTA treatment group. In PPTA group, PPTA was injected via the femoral vein immediately after reperfusion, and ischemia/reperfusion control group received saline injection. In 6 guinea pigs from each group, the cochlear tissues were removed after 24 h of reperfusion for examination of expressions of IL-1β and TNF-α mRNA by real-time PCR, and the rest animals were used for immunohistochemical detection of Fas protein.
RESULTSCompared with those of normal group and blank control group, the expressions of IL-1β and TNF-β mRNA increased significantly after cochlear ischemia/reperfusion (P<0.001), but were lowered significantly by PPTA (P<0.001). Positive expression of Fas protein expression was detected in the Corti organ, spiral ganglion and stria vascularis in ischemia/reperfusion control group with significantly higher IOD values than those of the other 3 groups (P<0.05). The IOD value showed no significant difference between PPTA-treated group, normal control group, and blank control group (P>0.05).
CONCLUSIONSPPTA can suppress the expression of Fas protein and IL-1β and TNF-β mRNAs in the cochlea of guinea pigs with cochlear ischemia/reperfusion. The protective effect of PPTA against cochlear ischemia/reperfusion is mediated probably by inhibition of inflammatory responses and cell apoptosis.
