Psoralen induces rat mesenchymal stem cell differentiation towards male germ cells in vitro.
- Author:
Qian WAN
1
,
2
;
Hua LU
;
Yanli DENG
;
Limei LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cell Proliferation; Cells, Cultured; Ficusin; pharmacology; Germ Cells; cytology; Male; Mesenchymal Stromal Cells; cytology; drug effects; Rats; Up-Regulation
- From: Journal of Southern Medical University 2013;33(12):1723-1726
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test the hypothesis that psoralen induces rat mesenchymal stem cells (RMSCs) to differentiate towards male germ cells.
METHODSRMSCs were induced by psoralen at the final concentration of 3.0 µg/ml, and the morphological changes of the cells were detected microscopically and the cell proliferation changes were measured by MTT assay. The expressions of 7 representative marker genes of male germ cells, namely Oct-4, Stra8, RVLG, SCP3, TNP2, Itgb1, and Itga6, were investigated in the induced cells by real-time quantitative PCR.
RESULTSRMSCs showed no obvious morphological changes after induction with 3.0 µg/ml psoralen for 72 h. Psoralen induction for 5 days did not significantly affect the proliferation of RMSCs; Psoralen induction for 72 h, however, significantly up-regulated the genes Oct-4, Stra8, SCP3, and Itgb1 (P<0.05) without affecting the expressions of the genes RVLG, TNP2, or Itga6 (P>0.05).
CONCLUSIONPsoralen may participate in the differentiation of RMSCs towards male germ cells.